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Description
Research Area
Images & Validation
−| Tested Applications | IHC, WB |
|---|---|
| Reactivity | Human, Mouse, Rat |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 30-207 of human CSF3 (NP_000750.1). |
| Target | CSF3 |
| Molecular Weight | Observed: 22kDa |
| Purification | Affinity purification |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | PBS with 0.02% sodium azide, 50% glycerol, pH 7.3. |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CSF3 Antibody [orb229759]
FC, ICC
Human
Mouse
Monoclonal
Unconjugated
G-CSF/CSF3 Rabbit Polyclonal Antibody [orb10702]
IF, IHC-Fr, IHC-P
Bovine, Porcine, Rat, Sheep
Human, Mouse
Rabbit
Polyclonal
Unconjugated
Human Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit [orb774890]
Human
15.63-1000 pg/mL
5.4 pg/mL
Mouse Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit [orb774891]
Mouse
7.82-500 pg/mL
2.8 pg/mL
Rat Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit [orb776358]
Rat
7.82-500 pg/mL
2.8 pg/mL

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Western blot analysis of extracts of various cell lines, using CSF3 antibody (orb1257171) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Immunohistochemistry of paraffin-embedded rat spleen using CSF3 antibody (orb1257171) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded human colon using CSF3 antibody (orb1257171) at dilution of 1:100 (40x lens).

Immunohistochemistry of paraffin-embedded mouse spleen using CSF3 antibody (orb1257171) at dilution of 1:100 (40x lens).
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CSF3 Antibody (orb1257171)
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