Cytokeratin 13 Antibody / KRT13

SKU: orb639774

Description

Cytokeratin 13 (KRT13) is the major acidic keratin, which together with KRT4, its basic partner, is expressed in the suprabasal layers of non-cornified stratified epithelia including tongue mucosa, esophagus, anal canal epithelium, tracheal epithelium, uterine cervix, and urothelium. Defects in the KRT13 gene are a cause of white sponge nevus of cannon (WSN), a rare autosomal dominant disorder, which predominantly affects non-cornified stratified squamous epithelia and is characterized by the presence of soft, white and spongy plaques in the oral mucosa. KRT13 has been used as a marker for non-keratinized squamous epithelium. It is also expressed in various squamous metaplasia, but it is down regulated in squamous dysplasia and squamous carcinoma.

Images & Validation

• Tested Applications: FACS, IF, IHC-P
• Dilution Range: Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml,Flow cytometry: 1-2/million cells
• Reactivity: Human
• Application Notes:
  Optimal dilution of the Cytokeratin 13 antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone No.: KRT13/2659
• Immunogen: A recombinant full-length human KRT13 protein was used as the immunogen for this Cytokeratin 13 antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Flow cytometry testing of permeabilized human HeLa cells with Cytokeratin 13 antibody (clone KRT13/2213); Red=isotype control, Blue= Cytokeratin 13 antibody.

IHC staining of FFPE human prostate carcinoma with Cytokeratin 13 antibody (clone KRT13/2213). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human tonsil with Cytokeratin 13 antibody (clone KRT13/2213). HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

SDS-PAGE analysis of purified, BSA-free Cytokeratin 13 antibody (clone KRT13/2213) as confirmation of integrity and purity.

Quick Database Links

UniProt

P13646