DAO Antibody

SKU: orb345021

Description

D-Amino Acid Oxidase antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IP, WB
• Dilution Range: ELISA: 1:5,000 - 1:20,000, IP: 1:100, WB: 1:500 - 1:5,000
• Reactivity: Porcine
• Application Notes:
  D-AMINO ACID OXIDASE antibody has been tested by ELISA and western blot. This product is assayed against 1.0 ug of D-Amino Acid Oxidase [Pig Kidney] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Goat) and ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:20,000 to 1:60,000 of the reconstitution concentration is suggested for this product.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: D-Amino Acid Oxidase [Pig Kidney]
• Target: DAO
• Purity: Anti-D-AMINO ACID OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified D-Amino Acid Oxidase [Pig Kidney]. Cross reactivity against D-Amino Acid Oxidase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

sheep anti-D-Amino Acid Oxidase Antibody, D-amino-acid oxidase, DAMOX, DAAO, DAO

Western Blot of Sheep anti-D-Amino Acid Oxidase Antibody. Lane 1: D-Amino Acid Oxidase. Lane 2: None. Load: 50 ng per lane. Primary antibody: D-Amino Acid Oxidase primary antibody at 1:1000 overnight at 4°C. Secondary antibody: Peroxidase sheep secondary antibody at 1:40000 for 30 min at RT. Block: orb348637 for 30 min at RT. Predicted/Observed size: 39 kDa, 39 kDa for D-Amino Acid Oxidase. Other band(s): None.

Quick Database Links

Gene Symbol

DAO

UniProt

P00371

RefSeq (Protein):NP_999231.1