DAZL Antibody

SKU: orb699733

Description

Specific for human DAZL, a member of the DAZ (deleted in azoospermia) family of RNA binding proteins. DAZL is expressed in fetal and adult testes and ovaries, and is believed to play a role in germ cell development. In adult germ cells, the expression of DAZL is localized to the cytoplasm. Mutations in this gene have been linked to severe spermatogenic failure and infertility in males. Clone DAZL/4253 recognizes DAZL at its C terminus, and is used in detection of DAZL expression by various methods.

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Reactivity: Human
• Application Notes:
  Optimal dilution of the DAZL antibody should be determined by the researcher.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone No.: DAZL/4253
• Immunogen: Amino acids CRVHHFRRSRAMLKSV from the human protein (C-terminal region) was used as the immunogen for the DAZL antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC staining of FFPE human testis with DAZL antibody. HIER: boil tissue sections in pH9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Western blot testing of human testis lysate with DAZL antibody. Predicted molecular weight: ~32 kDa.

SDS-PAGE analysis of purified, BSA-free DAZL antibody as confirmation of integrity and purity.

Quick Database Links

UniProt

Q92904