ddATP

SKU: orb65197

Description

ddATP (2',3'-Dideoxyadenosine-5'-triphosphate, Lithium salt) is a high-purity chain-terminating nucleotide analog widely used in DNA sequencing, primer extension, and molecular biology research. As a modified form of dATP, ddATP is enzymatically incorporated into DNA strands but lacks the essential 3'-OH group, resulting in immediate termination of DNA synthesis. This property makes it an essential reagent for Sanger sequencing and controlled DNA polymerization studies.

Images & Validation

• Application Notes:
  Spectroscopic Propertie: λmax 259 nm, ε 15.1 L mmol-1 cm-1 (Tris-HCl, pH 7.5).

Key Properties

• CAS Number: 24027-80-3
• MW: Theoretical MW: 475.18 g/mol (free acid); Detected MW: 475.01 g/mol (free acid)
• Purity: ≥ 98% (HPLC)
• Formula: C₁₀H₁₆N₅O₁₁P₃
• SMILES: OP(=O)(O)OP(=O)(O)OP(=O)(O)OC[C@H]1O[C@@H](n2cnc3c(N)ncnc23)CC1

Storage & Handling

• Storage: Store at -20°C. Short term exposure (up to 1 week cumulative) to ambient temperature possible.
• Expiration Date: 12 months from date of receipt.
• Hazard Information: Non-Toxic
• Disclaimer: For research use only

Alternative Names

2',3'-Dideoxyadenosine-5'-triphosphate, Lithium salt

Chemical structure of ddATP (Small Molecules)