DDB1 Recombinant Mouse Monoclonal Antibody

SKU: orb1173015

Description

DDB1 Recombinant Mouse Monoclonal Antibody

Research Area

Epigenetics & Chromatin

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-1000 (Human), IHC-P (Human, Mouse, Rat)=1:100-500, IHC-F (Human, Mouse, Rat)=1:100-500, IF (Human, Mouse, Rat)=1:100-500
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Recombinant
• Isotype: IgG2a, Kappa
• Clone No.: BC1J6
• Target: DDB1
• Molecular Weight: 127 kDa
• Purification: Affinity purified by Protein G
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DDBA; UV-DDB1; WHIKERS; XAP1; XPCE; XPE; XPE-BF; 127kDa; p127-Ddb1; DDB1_BOVIN; DDB1; Damage-specific DNA-binding protein 1; DDB1_HUMAN; DDB p127 subunit; DNA damage-binding protein a (DDBa); HBV X-associated protein 1 (XAP-1); UV-damaged DNA-binding factor; UV-damaged DNA-binding protein 1 (UV-DDB 1); XPE-binding factor (XPE-BF); Xeroderma pigmentosum group E-complementing protein (XPCe); DDB1_MOUSE; DDB1_RAT; damage specific DNA binding protein 1; damage-specific DNA binding protein 1 (127kD)

Blocking buffer: 5% NFDM/TBST, Primary ab Dilution: 1:1000, Primary ab incubation condition: 4°C overnight, Secondary ab: Goat Anti-Mouse IgG H&L (HRP), Lysate: HeLa, 293, MCF-7, Raw264.7, Protein loading quantity: 20 µg, Exposure time: 30 s, Predicted MW: 127 kDa, Observed MW: 127 kDa.

Tissue: Human liver cancer, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173015.

Tissue: Mouse liver, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173015.

Tissue: Rat colon, Section type: Formalin fixed & Paraffin-embedded section, Retrieval method: High temperature and high pressure, Retrieval buffer: Tris/EDTA buffer, pH9.0 Primary ab Dilution: 1:100, Primary ab incubation condition: 1 hour at room temperature, Secondary ab: SP Kit (Mouse), Counter stain: Hematoxylin (Blue), Comment: Color brown is the positive signal for orb1173015.

Quick Database Links

Gene Symbol

DDB1

UniProt

Q16531