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Description
Images & Validation
−| Tested Applications | ICC, IP, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try at WB: 1/100. Detects a band of approximately 160 kDa (predicted molecular weight: 122 kDa). |
| Reactivity | Human |
| Application Notes |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG1 |
| Clone No. | 10D2 |
| Immunogen | N terminal amino acids 37-164 of Human Desmoglein 2 fused to a proprietary tag. Sequence used LLPKHPHLVRQKRAWITAPVALREGEDLSKKNPIAKIHSDLAEERGLKITYKYTGKGITE PPFGIFVFNKDTGELNVTSILDREETPFFLLTGYALDARGNNVEKPLELRIKVLDINDNEPVFTQDVF |
| Target | Desmoglein 2 |
| Purification | Protein A Affinity Chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antibody containing PBS + 0.1% sodium azide |
| Concentration | 1mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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Western blot analysis of HaCat keratinocyte cells using Desmoglein 2 antibody
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Protocol Information
Desmoglein 2/DSG2 Monoclonal Antibody (orb76611)
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