DFFA Antibody

SKU: orb1239883

Description

DFFA Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, ICC, IF, WB
• Reactivity: Human
• Application Notes:
  DFF45 antibody can be used for detection of DFF45 and one of the cleaved fragments of DFF45 by Western blot at at 0.5 - 1 μg/mL and for immunoprecipitation. A 45 kDa band can be detected in non-apoptotic cells. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: DFF45 antibody was raised against a 19 amino acid peptide near the carboxy terminus of human DFF45.The immunogen is located within the last 50 amino acids of DFF45.
• Target: DFFA
• Molecular Weight: 45 kDa
• Purification: DFF45 Antibody is DEAE purified.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: DFF45 Antibody is supplied in PBS containing 0.02% sodium azide.
• Concentration: 1 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DFF45 Antibody: DFF1, ICAD, DFF-45, DFF1, DFF45, H13, DNA fragmentation factor subunit alpha, DNA fragmentation factor 45 kDa subunit

Western blot analysis of DFF45 in HeLa (H), Jurkat (J), A431 (A), and K562 (K) whole cell lysate with DFF45 antibody at 1:1000 dilution.

Immunocytochemistry of DFF45 in HeLa cells with DFF45 antibody at 5 µg/mL.

Immunofluorescence of DFF45 in Hela cells with DFF45 antibody at 20 µg/mL. Green: DFF45 Antibody (orb1239883) Blue: DAPI staining.

Quick Database Links

Gene Symbol

DFFA

UniProt

O00273

RefSeq (Protein):NP_004392