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DGAT1 Antibody

SKU: orb1539382

Description

DGAT1 Antibody

Research Area

Signal Transduction

Images & Validation

Tested ApplicationsELISA, IHC, IHC-P, WB
Dilution RangeIHC, IHC-P (5 µg/ml), Peptide-ELISA (1:128000), WB (0.5 - 2 µg/ml)
ReactivityBovine, Canine, Goat, Human, Monkey, Mouse, Porcine, Rabbit, Rat, Sheep
Application Notes
Further information: Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 50+55kD bands observed in Mouse Duodenum lysates (calculated MW of 56.8kD according to Mouse NP_034176.1). Recommended concentration: 0.5-2 ug/ml.

Key Properties

HostGoat
ClonalityPolyclonal
ImmunogenPeptide with sequence RCHRLQDSLFSSD, from the internal region of the protein sequence according to NP_036211.2.
TargetDGAT1
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesTBS, pH 7.3, 0.02% Sodium Azide, 0.5% rAlbumin
Concentration0.5 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

DGAT1, ACAT-related gene product 1, ACAT related gene product 1, ARGP1, ARAT, DGAT, Diglyceride acyltransferase, AGRP1

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DGAT1 Antibody

DGAT1 antibody (0.5 ug/ml) staining of Mouse Duodenum lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

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Protocol Information

WB
Western Blot (IB, immunoblot)
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IHC
Immunohistochemistry
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IHC-P
Immunohistochemistry Paraffin
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ELISA
Enzyme-linked Immunosorbent Assay (EIA)
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DGAT1 Antibody (orb1539382)

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Available Sizes

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50 μg
$ 740.00
Free Secondary Antibody (20 ul)0/0

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