DNCLI1 Rabbit Polyclonal Antibody

SKU: orb312221

Description

DNCLI1 Rabbit Polyclonal Antibody

Images & Validation

• Tested Applications: IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500
• Reactivity: Human, Mouse
• Predicted Reactivity: Gallus, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human DNCLI1 (451-523/523aa)
• Target: DYNC1LI1
• Molecular Weight: 57 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: Preservative: 0.02% Proclin300, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

DLC-A; DLIC-1; DNCLI1; LIC1; 1110053F02Rik; Dlic1; Dnclic1; LIC-1; DC1L1_HUMAN; DYNC1LI1; Dynein light chain A (DLC-A); Dynein light intermediate chain 1, cytosolic (DLIC-1); DC1L1_MOUSE; Dynein light intermediate chain 1, cytosolic; DC1L1_RAT; dynein cytoplasmic 1 light intermediate chain 1; dynein, cytoplasmic, light intermediate polypeptide 1

Sample: Heart (Mouse) Lysate at 40 ug, Primary: Anti-DNCLI1 (orb312221) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 57 kD, Observed band size: 57 kD.

Tissue/Cell: Human cervical cancer, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-DNCLI1 Polyclonal Antibody, Unconjugated (orb312221) 1:500, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

DYNC1LI1

UniProt

Q9Y6G9