DRD1 Rabbit Polyclonal Antibody

SKU: orb183263

Description

DRD1 Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: FC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:100-500, IHC-F=1:100-500, IF=1:100-500, Flow-Cyt=1μg/Test
• Reactivity: Human, Rat
• Predicted Reactivity: Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human Dopamine Receptor D1 (11-100/446aa)
• Target: DRD1
• Molecular Weight: 60 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

D1R; DADR; DRD1A; C030036C15Rik; Drd-1; Gpcr15; D1a; DRD1_HUMAN; DRD1; Dopamine D1 receptor; DRD1_MOUSE; DRD1_RAT; dopamine receptor D1

Cell: SH-SY5Y, Concentration: 1:100, Host/Isotype: Rabbit/IgG, Flow cytometric analysis of Rabbit IgG isotype control (Cat#: orb183263) on SH-SY5Y (green) compared with control in the absence of primary antibody (blue) followed by Alexa Fluor 488-conjugated goat anti-rabbit IgG (H+L) secondary antibody.

Sample: A431 Cell (Human) Lysate at 40 ug, HepG2 Cell (Human) Lysate at 40 ug, Primary: Anti-Dopamine Receptor D1 (orb183263) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50kD, Observed band size: 60kD.

Sample: A549 Cell (Human) Lysate at 40 ug, Primary: Anti-Dopamine Receptor D1 (orb183263) at 1/300 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 50kD, Observed band size: 60kD.

Tissue/Cell: rat brain tissue, 4% Paraformaldehyde-fixed and paraffin-embedded, Antigen retrieval: citrate buffer (0.01M, pH 6.0), Boiling bathing for 15 min, Block endogenous peroxidase by 3% Hydrogen peroxide for 30 min, Blocking buffer (normal goat serum) at 37°C for 20 min, Incubation: Anti-Dopamine Receptor D1 Polyclonal Antibody, Unconjugated (orb183263) 1:200, overnight at 4°C, followed by conjugation to the secondary antibody and DAB staining.

Quick Database Links

Gene Symbol

DRD1

UniProt

P21728