E-cadherin (Intracellular) Rabbit Polyclonal Antibody

SKU: orb1294381

Description

Rabbit polyclonal antibody to E-cadherin antibody (Intracellular domain)

Images & Validation

• Tested Applications: IF, IHC, WB
• Dilution Range: Western Blot 1:1000, Immunofluorescence 1:100-1:500, Immunohistochemistry (Paraffin) 1:100-1:200
• Reactivity: Human, Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: Rabbit-IgG
• Immunogen: Synthetic peptide / Intracellular domain corresponding to Human E-cadherin
• Target: E-cadherin (Intracellular)
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 100mM Tris Glycine, 1% rAlbumin, 20% Glycerol (pH7). 0.025% ProClin 300 was added as a preservative
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Anti-E cadherin antibody at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294381 overnight at 4°C.

Anti-E cadherin antibody at 1/1000 dilution. Lysates at 50 µg per lane. This blot was produced using a 12% SDS-PAGE. The Nitrocellulose membrane was then blocked for an hour before being incubated with orb1294381 overnight at 4°C. N-cadherin antibody (orb1294388).

Application: Immunocytochemistry (ICC), Sample: Mixture cell, Antibody: E-cadherin antibody (orb1294381).

Application: Immunofluorescence (ICC/IF), Sample: H1299, Permeabilization: 0.2% Triton X-100, Blocking: 3% BSA blocking for 1 hour, Temperature: RT Fixative: 4% PFA.

Application: Immunohistochemistry (FFPE), Sample: Human Cancer Tissue sectio ns, Antigen retrieval: Heat mediated Tris - Buffer pH9.

Immunofluorescence of paraffin-embedded sections. Cell: mouse colon, DAPI: 1-300, Green: E-cadherin (orb1294381): 1-300 Anti-rabbit 488: 1-300.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-cadherin with orb1294381 at 1/100.

Immunohistochemical analysis of paraffin embedded Human cancer tissue labeling E-cadherin with orb1294381 at 1/100.

Immunohistochemical analysis of paraffin embedded Human colorectal cancer tissue labeling E-cadherin with orb1294381 at 1/100.

Immunohistochemical analysis of paraffin embedded Human colorectal cancer tissue labeling E-cadherin with orb1294381 at 1/100.

Quick Database Links

Gene Symbol

E-cadherin (Intracellular)