EIF6 Antibody

SKU: orb1258453

Description

EIF6 Antibody

Research Area

Cell Biology, Neuroscience

Images & Validation

• Tested Applications: IF, WB
• Reactivity: Human
• Application Notes:
  WB: 1:500 - 1:2000IF: 1:50 - 1:200

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human EIF6 (NP_852133.1).
• Target: EIF6
• Molecular Weight: Observed: 27kDa
• Purification: Affinity purification
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.
• Concentration: batch dependent
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

CAB, EIF3A, ITGB4BP, b(2)gcn, eIF-6, p27(BBP), p27BBP, eukaryotic translation initiation factor 6, B4 integrin interactor, eukaryotic translation initiation factor 3A, p27 beta-4 integrin-binding protein

Western blot analysis of extracts of various cell lines, using EIF6 antibody (orb1258453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Immunofluorescence analysis of MCF-7 cells using EIF6 antibody (orb1258453). Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of Hela cells using EIF6 antibody (orb1258453) at dilution of 1:50. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2OS cells using EIF6 antibody (orb1258453) at dilution of 1:50. Blue: DAPI for nuclear staining.

Quick Database Links

Gene Symbol

EIF6

UniProt

P56537