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Description
Images & Validation
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| Tested Applications | EM, ICC, WB |
|---|---|
| Dilution Range | Optimal antibody dilution should be determined by titration, however as a guideline try WB: 1/1000. Predicted molecular weight: 30 kDa.ICC/IF: 1/100.Electron MicroscopyEM: 1/50. (Rat) |
| Reactivity | Hamster, Mouse, Rat |
Key Properties
−| Host | Sheep |
|---|---|
| Clonality | Monoclonal |
| Clone No. | APS20 antisera |
| Immunogen | Synthetic peptide: MSKSFRRPGT SLVDADDTFH HQVRDDIFSS SEEEGKDRER PIYGRDSAYQ SIAEYRPISN VSRSSLGLSY, corresponding to internal sequence amino acids 114-183 of Rat Emerin, with N terminal proprietary tag. (P50402) |
| Target | Emerin |
| Purification | Affinity purified on GST-sepharose column with attached antigen |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Purified antiserum in (PBS + 10% gelatin) + 0.1% sodium azide |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−EDMD antibody;EMD antibody;Emery Dreifuss muscular dystrophy antibody;STAantibody
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Confocal immunofluorescence analysis of paraffin-embedded cancer cells using Emerin antibody
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Protocol Information
WB
Western Blot (IB, immunoblot)
ICC
Immunocytochemistry
EM
Electron Microscopy
Emerin Antibody (orb76620)
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Free Secondary Antibody (20 ul)0/0
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