EP4 Rabbit Polyclonal Antibody

SKU: orb1063819

Description

EP4 Rabbit Polyclonal Antibody

Research Area

G Protein-Coupled Receptors, GPCR Signaling Pathway, Prostaglandin

Images & Validation

• Tested Applications: FC, WB
• Dilution Range: WB=1:500-2000, Flow-Cyt=2ug/Test
• Reactivity: Mouse

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from mouse EP4 (1-100/513aa)
• Target: PTGER4
• Molecular Weight: 55 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

EP4; EP4R; Ptgerep4; Ptger; PGE2R-EP4; PE2R4_HUMAN; PTGER4; PGE receptor EP4 subtype; PGE2 receptor EP4 subtype; Prostanoid EP4 receptor; PTGER2; PE2R4_MOUSE; PE2R4_RAT; prostaglandin E receptor 4; prostaglandin E receptor 4 (subtype EP4)

Blank control: Ctll-2. Primary Antibody (green line): Rabbit Anti-EP4 antibody (orb1063819), Dilution: 2 ug/Test, Secondary Antibody: Goat anti-rabbit IgG-FITC, Dilution: 0.5 ug/Test. Protocol, The cells were incubated in 5% BSA to block non-specific protein-protein interactions for 30 min at room temperature. Cells stained with Primary Antibody for 30 min at room temperature. The secondary antibody used for 40 min at room temperature. Acquisition of 20000 events was performed.

Sample: Lane 1: Mouse Uterus tissue lysates, Lane 2: Mouse Small intestine tissue lysates, Lane 3: Mouse Kidney tissue lysates, Lane 4: Mouse Prostate tissue lysates, Primary: Anti-EP4 (orb1063819) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 53 kD, Observed band size: 55 kD.

Quick Database Links

Gene Symbol

PTGER4

UniProt

P32240