EpCAM Antibody / Extracellular domain

SKU: orb750171

Description

EpCAM may act as a physical homophilic interaction molecule between intestinal epithelial cells (IECs) and intraepithelial lymphocytes (IELs) at the mucosal epithelium for providing immunological barrier as a first line of defense against mucosal infection. [UniProt]

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: IF, IHC-P, WB
• Dilution Range: Western blot: 1-2ug/ml,Immunofluorescence: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Reactivity: Human
• Application Notes:
  The concentration stated for each application is a general starting point. Variations in protocols, secondaries and substrates may require the EpCAM antibody to be titered up or down for optimal performance.

Key Properties

• Antibody Type: Primary Antibody
• Host: Mouse
• Clonality: Monoclonal
• Isotype: Mouse IgG2b, kappa
• Clone No.: EPM17-4
• Immunogen: Human recombinant partial protein (extracellular portion) was used as the immunogen for this EpCAM antibody.
• Purification: Protein G affinity chromatography
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin and 0.05% sodium azide
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

IHC testing of FFPE human fallopian tube with EpCAM antibody (clone EPM17-4). Staining of FFPE tissue requires boiling sections in pH9 10 mM Tris with 1 mM EDTA for 10-20 min followed by cooling at RT for 20 min.

Western blot testing of human lung lysate with EpCAM antibody (clone EPM17-4). Expected molecular weight: ~35 kDa (unmodified), 40-43 kDa (glycosylated).

Immunofluorescent staining of human MCF7 cells with EpCAM antibody (clone EPM17-4, green) and Reddot nuclear stain (red).

Quick Database Links

UniProt

P16422