F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, HRP conjugated

SKU: orb1151905

Description

The F(ab')₂ Fragment Goat Anti-Mouse IgG H&L, HRP conjugated is an HRP conjugated polyclonal antibody suitable for ELISA, IHC-Fr, IHC-P, WB. This product reacts with Mouse samples.

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC-Fr, IHC-P, WB
• Reactivity: Mouse

Key Properties

• Antibody Type: Secondary Antibody
• Host: Goat
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Native Mouse IgG
• Purification: Affinity purified by Protein G
• Conjugation: HRP

Storage & Handling

• Storage: Store at -20°C for one year. Avoid repeated freeze/thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 10 mM TBS (pH=7.4) with 1% rAlbumin, 0.03% Proclin300 and 50% glycerol.
• Concentration: 1.0 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

F(ab)2 Fragment Goat Anti-Mouse IgG H&L (HRP); F(ab)2 Fragment Goat Anti-Mouse IgG H&L/HRP; F(ab)2 Fragment Goat Anti-Mouse IgG(H+L);

Paraformaldehyde-fixed, paraffin embedded (rat cerebellum), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (S100B) Monoclonal Antibody, Unconjugated (orb500644) at 1:200 overnight at 4°C, An HRP-conjugated secondary (orb1151905, 1:300 dilution) was used for 20min at 37°C, DAB was used as the chromogen.

Paraformaldehyde-fixed, paraffin embedded (rat pancreas), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (GLP-1 (1G9)) Monoclonal Antibody, Unconjugated (orb10721) at 1:200 overnight at 4°C, An HRP-conjugated secondary (orb1151905, 1:300 dilution) was used for 20min at 37°C, DAB was used as the chromogen.