Fatty Acid Synthase Recombinant Rabbit Monoclonal Antibody

SKU: orb704303

Description

Fatty Acid Synthase Recombinant Rabbit Monoclonal Antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: FC, ICC, IF, IHC-Fr, IHC-P, WB
• Dilution Range: WB=1:500-2000, IHC-P=1:50-200, IHC-F=1:50-200, ICC/IF=1:50-200, IF=1:50-200, Flow-Cyt=1:50-100
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Recombinant
• Isotype: IgG
• Clone No.: B1D8
• Immunogen: A synthesized peptide derived from human Fatty acid synthase (2450-2511aa)
• Target: FASN
• Molecular Weight: 290 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

FAS; OA-519; SDR27X1; A630082H08Rik; FAS_CHICK; FASN; 2.3.1.85; FAS_BOVIN; FAS_HUMAN; Type I fatty acid synthase; FAS_MOUSE; FAS_RAT; Type I FAS; fatty acid synthase; short chain dehydrogenase/reductase family 27X, member 1; [Acyl-carrier-protein] S-acetyltransferase; [Acyl-carrier-protein] S-malonyltransferase; 3-oxoacyl-[acyl-carrier-protein] synthase; 3-oxoacyl-[acyl-carrier-protein] reductase; 3-hydroxyacyl-[acyl-carrier-protein] dehydratase; Enoyl-[acyl-carrier-protein] reductase; Acyl-[acyl-carrier-protein] hydrolase

Paraformaldehyde-fixed, paraffin embedded (human breast carcinoma), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Fatty Acid Synthase) Monoclonal Antibody, Unconjugated (orb704303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Paraformaldehyde-fixed, paraffin embedded (human breast), Antigen retrieval by microwaving in EDTA buffer (Ph8.0) for 5 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (Fatty Acid Synthase) Monoclonal Antibody, Unconjugated (orb704303) at 1:200 overnight at 4°C, followed by operating according to SP Kit (Rabbit) instructionsand DAB staining.

Sample: Lane 1: Human A549 cell lysates, Lane 2: Human MCF-7 cell lysates, Primary: Anti-Fatty Acid Synthase (orb704303) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 273 kDa, Observed band size: 250 kDa.

Quick Database Links

Gene Symbol

FASN

UniProt

P49327