FLG Antibody (Biotin)

SKU: orb751156

Description

Mouse monoclonal antibody against FLG conjugated to Biotin

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: IHC
• Dilution Range: Immunohistochemistry (Formalin-fixed) (2-4ug/ml for 30 minutes at RT),(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95 °C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Reactivity: Human
• Application Notes:
  Positive Control: U-251-MGcells.Human skin

Key Properties

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b
• Clone No.: FLG/1563
• Immunogen: Recombinant human Filaggrin protein fragment (around aa 998-1104) (exact sequence is proprietary)
• Target: FLG
• Source/Expression System: Bioreactor
• Molecular Weight: 26-45kDa (Processed); 350kDa (Profilaggrin)
• Purification: Protein A
• Conjugation: Biotin

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: 200ug/ml of Ab Purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Concentration: Purified Ab conjugated to Biotin
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

Anti-ATOD2 antibody, anti-Epidermal Filaggrin antibody, anti-Filaggrin antibody, anti-Filaggrin precursor antibody, anti-Fillagrin antibody, anti-FLG antibody, anti-Profilaggrin antibody

Analysis of Protein Array containing more than 19000 full-length human proteins using Filaggrin Mouse Monoclonal Antibody (FLG/1563). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.

Formalin-fixed, paraffin-embedded human skin stained with Biotin-conjugated FilaggrinMouse Monoclonal Antibody(FLG/1563).

SDS-PAGE Analysis of Purified FilaggrinMouse Monoclonal Antibody(FLG/1563) (unconjugated). Confirmation of Integrity and Purity of Antibody.

Quick Database Links

Gene Symbol

FLG

UniProt

P20930

NCBI Gene ID

2312