FOXO3A Rabbit Polyclonal Antibody

SKU: orb10671

Description

FOXO3A Rabbit Polyclonal Antibody

Research Area

Cell Biology

Images & Validation

• Tested Applications: WB
• Dilution Range: WB=1:500-2000
• Reactivity: Human, Mouse
• Predicted Reactivity: Mouse, Rat

Key Properties

• Antibody Type: Primary Antibody
• Host: Rabbit
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: KLH conjugated synthetic peptide derived from human FOXO3A (201-300/673aa)
• Target: FOXO3
• Molecular Weight: 85 kDa
• Purification: Affinity purified by Protein A
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Form/Appearance: Liquid
• Buffer/Preservatives: 0.01M TBS (pH7.4) with 1% rAlbumin, 0.02% Proclin300 and 50% Glycerol.
• Concentration: 1mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

AF6q21; FKHRL1; FKHRL1P2; FOXO2; FOXO3A; 1110048B16Rik; 2010203A17Rik; Fkhr2; FOXO3_HUMAN; FOXO3; AF6q21 protein; Forkhead in rhabdomyosarcoma-like 1; FOXO3_MOUSE; forkhead box O3

Paraformaldehyde-fixed, paraffin embedded (Rat rectum tissue), Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15 min, Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes, Blocking buffer (normal goat serum) at 37°C for 30 min, Antibody incubation with (FOXO3A) Polyclonal Antibody, Unconjugated (orb10671) at 1:400 overnight at 4°C, followed by a conjugated secondary for 20 minutes and DAB staining.

Sample: Lane 1: Mouse Testis tissue lysates, Lane 2: Mouse Uterus tissue lysates, Lane 3: Rat Testis tissue lysates, Lane 4: Human MCF-7 cell lysates, Lane 5: Human DU145 cell lysates, Primary: Anti-FOXO3A (orb10671) at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution, Predicted band size: 74 kDa, Observed band size: 90 kDa.

Quick Database Links

Gene Symbol

FOXO3

UniProt

O43524