Furin

SKU: orb1176572

Description

Furin (proprotein convertase subtilisin/kexin type 3, PCSK3, PACE) belongs to the proprotein convertases involved in the proteolytic maturation of many proteins in the secretory pathway. In addition to cellular substrates, furin activates the proteins of many bacterial and viral pathogens, including the Spike-protein of the severe acute respiratory virus 2 (SARS-CoV-2). Recombinant human furin is expressed in the human HEK293S cell line facilitating homogenous glycosylation. Active furin is selectively purified using inhibitor based affinity purification. The protein is suited for enzymatic assays, activation of furin's substrate proteins and protein crystallography. Ca2+ is crucial for activity; avoid calcium chelators like citrate, EDTA or EGTA in buffers. For dilution below 0.1 mg/ml include detergents (e.g. 0.2% Triton-X-100) in buffers. The activity is defined by the amount of furin (µg) that releases 1 pmol AMC from Pyr-Arg-Thr-Lys-Arg-AMC in 1 minute (in 100 mM Hepes/NaOH pH 7.0, 2 mM, 0.2% Triton-X-100 at 37°C with 200 µM Pyr-Arg-Thr-Lys-Arg-AMC).

Key Properties

• Source: human cell line HEK293S
• Biological Origin: human
• MW: Theoretical MW: 52.3 kDa
• Purity: > 90% (SDS-PAGE)

Storage & Handling

• Storage: store at -80 °C. avoid freeze/thaw cycles.
• Form/Appearance: liquid (supplied in 10 mM Hepes/NaOH pH 7.4, 100 mM NaCl, 2mM CaCl2)
• Expiration Date: 6 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

Proprotein Convertase Subtilisin/Kexin Type 3 (PCSK3), human, recombinant, human cell line HEK293S

Quick Database Links

UniProt

P09958