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Description
Research Area
Images & Validation
−| Tested Applications | ELISA, NeA, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant mG-CSF. |
| Target | Csf3 |
| Purification | Anti-mG-CSF specific antibody was purified by affinity chromatography employing immobilized mG-CSF matrix. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Concentration | batch dependent |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Similar Products
−CSF3 Antibody [orb229759]
FC, ICC
Human
Mouse
Monoclonal
Unconjugated
G-CSF/CSF3 Rabbit Polyclonal Antibody [orb10702]
IF, IHC-Fr, IHC-P
Bovine, Porcine, Rat, Sheep
Human, Mouse
Rabbit
Polyclonal
Unconjugated
Human Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit [orb774890]
Human
15.63-1000 pg/mL
5.4 pg/mL
Mouse Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit [orb774891]
Mouse
7.82-500 pg/mL
2.8 pg/mL
Rat Colony Stimulating Factor 3, Granulocyte (GCSF) ELISA Kit [orb776358]
Rat
7.82-500 pg/mL
2.8 pg/mL

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To detect mG-CSF by sandwich ELISA (using 100 ul/well antibody solution) a concentration of 0.5 - 2.0 ug/ml of this antibody is required. This antigen affinity purified antibody, in conjunction with Anti-Murine G-CSF (orb1272528) as a detection antibody, allows the detection of at least 0.2 - 0.4 ng/well of recombinant mG-CSF.

To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

To detect mG-CSF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 ug/ml. Used in conjunction with compatible secondary reagents the detection limit for recombinant mG-CSF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.
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Protocol Information
Csf3 Antibody (orb1272529)
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