gox Antibody

SKU: orb345060

Description

Glucose Oxidase antibody

Research Area

Metabolism Research

Images & Validation

• Tested Applications: DOT, ELISA, WB
• Dilution Range: ELISA: 1:5,500 - 1:25,000, WB: 1:500 - 1:3,000
• Reactivity: Fungi
• Application Notes:
  Glucose Oxidase antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Glucose Oxidase [Aspergillus niger] in a standard ELISA using Peroxidase conjugated Affinity Purified anti-Sheep IgG [H&L] (Rabbit) and (ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:2,000 to 1:10,000 of the reconstitution concentration is suggested for this product.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Glucose Oxidase [Aspergillus niger]
• Target: gox
• Purity: Anti-GLUCOSE OXIDASE is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Form/Appearance: Lyophilized
• Buffer/Preservatives: Preservative: 0.01% (w/v) Sodium Azide. Stabilizer: None; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

sheep anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Dot Blot results of Sheep Anti-Glucose Oxidase Antibody. Dots are Glucose Oxidase at (1) 100 ng, (2) 33.3 ng, (3) 11.1 ng, (4) 3.70 ng, (5) 1.23 ng. Blocking: orb348637 for 30 min at RT. Primary Antibody: Sheep Anti-Glucose Oxidase Antibody at 10 µg/ml for 1hr at RT. Secondary Antibody: Donkey Anti-Sheep-HRP (p/n orb347953) at 1:40000 for 30 min at RT.

Quick Database Links

Gene Symbol

gox

UniProt

P13006