gox Antibody (HRP)

SKU: orb345067

Description

Glucose Oxidase antibody (Peroxidase)

Research Area

Metabolism Research

Images & Validation

• Tested Applications: DOT
• Dilution Range: WB: User Optimized
• Reactivity: Fungi
• Application Notes:
  Anti-Glucose Oxidase peroxidase conjugated antibody has been tested by dot blot and is suitable to be assayed against 1.0 ug of Glucose Oxidase [Aspergillus niger] in a standard capture ELISA using ABTS (2,2’-azino-bis-[3-ethylbenthiazoline-6-sulfonic acid]) as a substrate for 30 minutes at room temperature. A working dilution of 1:1,000 to 1:3,000 of the reconstitution concentration is suggested for this product.

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Key Properties

• Antibody Type: Primary Antibody
• Host: Sheep
• Clonality: Polyclonal
• Isotype: IgG
• Immunogen: Glucose Oxidase [Aspergillus niger]
• Target: gox
• Purity: Anti-Glucose Oxidase (Sheep) antibody is an IgG fraction antibody purified from monospecific antiserum by a multi-step process which includes delipidation, salt fractionation and ion exchange chromatography followed by extensive dialysis against the buffer stated above. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Sheep Serum as well as purified and partially purified Glucose Oxidase [Aspergillus niger]. Cross reactivity against Glucose Oxidase from other tissues and species may occur but have not been specifically determined.
• Conjugation: HRP

Storage & Handling

• Storage: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 µL). To minimize loss of volume dilute 1:10 by adding 225 µL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.
• Form/Appearance: Liquid (sterile filtered)
• Buffer/Preservatives: Preservative: 0.01% (w/v) Gentamicin Sulfate. Do NOT add Sodium Azide!. Stabilizer: 10 mg/mL Bovine Serum Albumin (rAlbumin) - Immunoglobulin and Protease free; Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2
• Concentration: 1.0 mg/mL
• Expiration Date: 12 months from date of receipt.
• Dry Ice Shipping: Please note: This product requires shipment on dry ice. A dry ice surcharge will apply.
• Disclaimer: For research use only

Alternative Names

sheep anti-Glucose Oxidase HRP conjugated Antibody, peroxidase conjugated sheep anti-Glucose Oxidase Antibody, Beta D Glucose Oxygen 1 Oxido Reductase antibody, Glucose oxidase (Precursor) antibody, Glucose Oxyhydrase antibody, GOD antibody

Dot Blot of Sheep Anti-GLUCOSE OXIDASE Antibody Peroxidase Conjugated. Lane 1: 100 ng Glucose Oxidase. Lanes 2-5: serial dilution 3 fold of Antigen. Primary Antibody: none. Secondary Antibody: Anti-Glucose Oxidase Peroxidase Conj'd (p/n orb345066) at 1:100 dilution of 1 mg/ml at RT for 30 minutes. Block: orb348637 at RT for 30 minutes.

Quick Database Links

Gene Symbol

gox

UniProt

P13006

RefSeq:P13006.1