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GYPA Antibody

SKU: orb1247965

Description

GYPA Antibody

Research Area

Immunology & Inflammation

Images & Validation

Tested ApplicationsELISA, IHC, WB
ReactivityHuman
Application Notes
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 18kDa band observed in Human Liver lysates (calculated MW of 16.4kDa according to NP_002090.2). Recommended concentration: 1-3µg/mL. Primary incubation was 1 hour.Immunohistochemistry:In paraffin embedded Human Kidney shows bright staining of erythrocytes. Recommended concentration: 2-4µg/mL.

Key Properties

Antibody TypePrimary Antibody
HostGoat
ClonalityPolyclonal
ImmunogenThe immunogen for this antibody is: C-SVEIENPETSDQ
TargetGYPA
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration500 ug/mL
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

GYPA, glycophorin A (includes MN blood group), HGNC:4702, GPA, GPErik, GPSAT, HGpMiV, HGpMiX, HGpMiXI, HGpSta(C), MN, MNS, Mi.V glycoprotein (24 AA), erythroid-lineage-specific membrane sialoglycoprotein, glycophorin A, glycophorin A, GPA, glycophorin Erik, glycophorin MiI, glycophorin MiV, glycophorin MiX, glycophorin SAT, glycophorin Sta type C, recombinant MiDR, recombinant glycophorin A-B Miltenberger-DR, CD235a, GpMiIII, HGpMiIII, glycophorin A (MN blood group), glycophorin MiIII

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GYPA Antibody

orb1247965 (1 ug/ml) staining of Human Liver lysate (35 ug protein in RIPA buffer). Detected by chemiluminescence.

GYPA Antibody

orb1247965 (2 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

UniProt Details

No UniProt data available

NCBI Reference Sequences

Associated Accession Numbers
Curated reference sequences for the gene transcript and protein product

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

GYPA Antibody (orb1247965)

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0.1 mg
$ 920.00
Free Secondary Antibody (20 ul)0/0

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