Alpha-synuclein (NACP140) Antibody

SKU: orb46817

Description

Goat polyclonal antibody to SNCA

Research Area

Neuroscience

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Porcine
• Application Notes:
  WB: Approx 17kDa band observed in Human Brain (Amygdala) lysates (calculated MW of 14.5kDa according to NP_000336.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Brain (Cerebellum). Recommended concentration: 5µg/ml.

Key Properties

• Clonality: Polyclonal
• Target: Alpha-synuclein (NACP140)
• Protein Sequence: KDQLGKNEEGAPQE
• Molecular Weight: 14.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration: 0.5 mg/ml
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NACP antibody, anti PARK1 antibody, anti PARK4 antibody, anti MGC110988 antibody

Western blot analysis of Human Amygdala lysate using SNCA antibody

Immunohistochemical staining of Human Cerebellum using SNCA antibody

Quick Database Links

Gene Symbol

Alpha-synuclein (NACP140)

NCBI Gene ID

6622