ARL2 Antibody

SKU: orb18406

Description

Goat polyclonal antibody to ARL2

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:1000, WB: 1-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine
• Application Notes:
  WB: Approx. 18kDa band observed in lysates of cell line A431 and approx. 20kDa in nuclear lysates of cell line HeLa (calculated MW of 20.9kDa according to NP_001658.2). Recommended concentration: 0.5-1µg/ml.Primary incubation 1 hour at room temperature.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm and nuclei of U2OS cells. Recommended concentration: 10µg/ml. Flow Cytometry: Flow cytometric analysis of A431 cells. Recommended concentration

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Key Properties

• Target: ARL2
• Protein Sequence: LDDISSRIFTAD
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

ARL2; ADP-ribosylation factor-like 2; ARFL2; ADP=ribosylation factor-like 2

orb18406 staining (2ug/ml) of U937 lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

Quick Database Links

Gene Symbol

ARL2