ATF2 Antibody

SKU: orb18311

Description

Goat polyclonal antibody to ATF2

Research Area

Cancer, DNA Damage, Epigenetics

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.3-1 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Experiments gave bands at approx 55kDa and 30kDa in lysates of Human Placenta and of cell line HeLa after 0.5 μg/ml antibody staining. These bands correspond to earlier findings in literature with different antibodies (Bailey et al , J Clin Endocrinol Metab. 2002 Apr;87(4):1717-28.; PMID: 11932306). This protein has a calculated MW of 54.5kDa according to NP_001871.2. Recommended concentration: 0.5-1.5 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: ATF2
• Protein Sequence: IVMAPSSQSQPSGS
• Molecular Weight: 54.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ATF2 antibody, anti activating transcription factor 2 antibody, anti HB16 antibody, anti CREB2 antibody, anti TREB7 antibody, anti CRE-BP1 antibody, anti cAMP responsive element binding protein 2 antibody, anti MGC111558 antibody, anti activating transcription factor 2 splice variant ATF2-var2 antibody, anti cAMP responsive element binding protein 2, formerly antibody

Western blot analysis of Human Placenta lysate using ATF2 antibody

Western blot analysis of HepG2 (Lane 1), K562 (Lane 2) and HeLa (Lane 3) nuclear lysates using ATF2 antibody

Quick Database Links

Gene Symbol

ATF2

NCBI Gene ID

1386