CBX5/HP1-Alpha Antibody

SKU: orb19140

Description

Goat polyclonal antibody to CBX5

Research Area

Epigenetics, Infectious Diseases

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:128000, WB: 0.03-0.1 μg/ml, IHC-P: 2-4 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: In paraffin embedded Human Uterus shows strong nuclear staining in the glandular epithelial cells. Recommended concentration, 2-3ug/ml.WB: Approx. 22kDa band observed in lysates of Human fibroblast cell lines A431 and HeLa (calculated MW of 22.2kDa according to NP_036249.1). Recommended concentration: 0.03-0.1 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: CBX5 / HP1-Alpha
• Protein Sequence: NKRKSNFSNSADDIK
• Molecular Weight: 22.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CBX5 antibody, anti HP1-ALPHA antibody, anti chromobox homolog 5 (HP1 alpha homolog, Drosophila) antibody, anti HGNC:1555 antibody, anti HP1 antibody, anti HP1Hs-alpha antibody, anti chromobox homolog 5 (Drosophila HP1 alpha) antibody, anti Heterochromatin protein-1 antibody, anti HP1A antibody, anti antigen p25 antibody, anti heterochromatin protein 1 homolog alpha antibody, anti heterochromatin protein 1-alpha antibody

Western blot analysis of A43N (A) and K562 (B) cell lysate using CBX5 antibody.

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using CBX5 antibody.

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using CBX5 antibody.

Quick Database Links

Gene Symbol

CBX5 / HP1-Alpha

NCBI Gene ID

23468