CDX2 Antibody

SKU: orb19201

Description

Goat polyclonal antibody to CDX2

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:4000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:4000.WB: Approx 38kDa band observed in Human Kidney and Liver lysates (calculated MW of 33.5kDa according to NP_001256.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Qualtrough et al Gut. 2002 Aug;51(2):184-90; PMID: 12117877). Recommended concentration: 1-3 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: CDX2
• Protein Sequence: EQLSPGGQRRNLCE
• Molecular Weight: 33.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CDX2 antibody, anti caudal type homeo box transcription factor 2 antibody, anti HGNC:1806 antibody, anti CDX-3 antibody, anti CDX3 antibody

orb19201 (1ug/ml) staining of Human Kidney lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical staining of Human Colon using CDX2 antibody.

Quick Database Links

Gene Symbol

CDX2

NCBI Gene ID

1045