CMG1/CCDC2/IFT74 Antibody

SKU: orb18684

Description

Goat polyclonal antibody to IFT74

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.2-0.6 μg/ml
• Reactivity: Human
• Predicted Reactivity: Rat

Key Properties

• Clonality: Polyclonal
• Target: CMG1 / CCDC2 / IFT74
• Protein Sequence: KTIVDALHSTSGN
• Molecular Weight: 69.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti IFT74 antibody, anti CCDC2 antibody, anti CMG-1 antibody, anti FLJ22621 antibody, anti MGC111562 antibody, anti intraflagellar transport 74 homolog (Chlamydomonas) antibody, anti capillary morphogenesis protein 1 antibody, anti coiled-coil domain containing 2 antibody, anti CMG1 antibody

orb18684 staining (0.3ug/ml) of 293 lysate (RIPA buffer, 35ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18684 (5ug/ml) staining of paraffin embedded Human Liver. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. Figure 2: orb18684 (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body

A)orb18684 (2.5mg/ml) staining of primary rat cortical neurons showed localization of IFT74 to vesicles in the cell body and along the neuronal processes. B) Control. (Data were kindly provided by Dr. Bryan Traynor.)

Quick Database Links

Gene Symbol

CMG1 / CCDC2 / IFT74

NCBI Gene ID

80173