Connexin 43/GJA1 Antibody

SKU: orb20237

Description

Goat polyclonal antibody to GJA1

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, FC, IF, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-1 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Canine, Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 40kDa band observed in Rat Brain lysates (calculated MW of 43.0kDa according to Human NP_000156.1 and to Rat NP_036699.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: Connexin 43 / GJA1
• Protein Sequence: QPFDFPDDNQNSKK
• Molecular Weight: 43
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GJA1 antibody, anti gap junction protein, alpha 1, 43kDa antibody, anti CX43 antibody, anti DFNB38 antibody, anti GJAL antibody, anti ODDD antibody, anti connexin 43 antibody, anti gap junction 43 kDa heart protein antibody, anti gap junction protein, alpha-like antibody

Western blot analysis of Human and Rat Heart lysate using GJA1 antibody

Western blot analysis of Human and Rat Heart lysate using GJA1 antibody

0.1 µg/mL staining of Human Cerebellum lysate (35 µg protein in RIPA buffer). Detected by chemiluminescence.

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and plasma membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic, plasma membrane and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Quick Database Links

Gene Symbol

Connexin 43 / GJA1

NCBI Gene ID

2697