CREB1 (aa96-109) Antibody

SKU: orb137115

Description

Goat polyclonal antibody to CREB1

Research Area

Stem Cell & Developmental Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine
• Application Notes:
  WB: Approx 45kDa band observed in nuclear lysates of cell lines Jurkat and NIH3T3 (calculated MW of 36.7kDa according to NP_604391.1). This molecular weight is routinely observed by other sources. Recommended concentration: 0.1-0.3µg/ml. Primary incubation wa. IHC: Paraffin embedded Human Prostate. Recommended concentration: 2.5µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

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Key Properties

• Clonality: Polyclonal
• Target: CREB1 (aa96-109)
• Protein Sequence: DSQESVDSVTDSQK
• Molecular Weight: 35.1; 36.7
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Concentration:

  0.1-0.3 μg/ml.

• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CREB1 antibody, anti cAMP responsive element binding protein 1 antibody, anti cAMP-response element-binding protein-1 antibody, anti cAMP-responsive element-binding protein 1 antibody, anti cyclic AMP-responsive element-binding protein 1 antibody, anti transactivator protein antibody

Western blot analysis of Jurkat (Lane 1) and NIH3T3 (Lane 2) nuclear lysates using CREB1 antibody

Immunohistochemical staining of Human Prostate using CREB1 antibody

Quick Database Links

Gene Symbol

CREB1 (aa96-109)

NCBI Gene ID

1385