CYP17A1 Antibody

SKU: orb19207

Description

Goat polyclonal antibody to CYP17A1

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.1-0.3 μg/ml, IHC-P: 3-5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Canine, Feline
• Application Notes:
  IHC: In paraffin embedded Human Adenal Gland shows strong granular staining of the cytoplasm in medulla cells. Recommended concentration, 3-5μg/ml.WB: Approx 60kDa band observed in human ovary lysates (calculated MW of 57.4kDa according to NP_000093.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: CYP17A1
• Protein Sequence: LFKDGDQKLEK
• Molecular Weight: 57.4
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CYP17A1 antibody, anti cytochrome P450, family 17, subfamily A, polypeptide 1 antibody, anti HGNC:2593 antibody, anti CPT7 antibody, anti CYP17 antibody, anti P450C17 antibody, anti S17AH antibody, anti cytochrome P450, family 17 antibody, anti cytochrome P450, subfamily XVII (steroid 17-alpha-hydroxylase), adrenal hyperplasia antibody, anti cytochrome p450 XVIIA1 antibody, anti steroid 17-alpha-hydroxylase/17,20 lyase antibody, anti steroid 17-alpha-monooxygenase antibody

CYP17A1 antibody (0.3 µg/ml) staining of human ovary lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Immunohistochemical analysis of formalin-fixed and paraffin embedded human adrenal gland tissue using CYP17A1 antibody

Quick Database Links

Gene Symbol

CYP17A1

NCBI Gene ID

1586