DIO2 Antibody

SKU: orb19555

Description

Goat polyclonal antibody to DIO2

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, FC, IF
• Dilution Range: ELISA: 1:128000, IHC-P: 2-3 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.IHC: Cryo-sectioned Mouse Brain (Hippocampus) shows staining in the granular layer of the dentate gyrus. Recommended concentration, 2-3ug/ml. Data obtained from anonymous customer.WB: Preliminary experiments gave an approx 65-70kDa band in Human Heart and Thyroid lysates after 0.05 μg/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.5kDa according to NP_NP_000784.2 and of 34.6kDa according to NP_001007024.1. The 65-70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates Have any further splice variants/modified forms been reported

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Key Properties

• Clonality: Polyclonal
• Target: DIO2
• Protein Sequence: EVKKHQNQEDRC
• Molecular Weight: 30.5; 34.6
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti DIO2 antibody, anti deiodinase, iodothyronine, type II antibody, anti 5DII antibody, anti D2 antibody, anti SelY antibody, anti TXDI2 antibody, anti thyroxine deiodinase, type II antibody, anti type 2 iodothyronine deiodinase antibody, anti type II iodothyronine deiodinaseN: type-II 5\1deiodinase antibody

orb19555 (2ug/ml) staining of PFA-fixed cryo-sectioned Mouse Hippocampus. Microwaved antigen retrieval with citrate buffer pH 4.5, HRP-staining.

Immunofluorescence analysis of HeLa cells using DIO2 antibody

Flow cytometric analysis of MCF7 cells using DIO2 antibody

Quick Database Links

Gene Symbol

DIO2

NCBI Gene ID

1734