FABP2 Antibody

SKU: orb19465

Description

Goat polyclonal antibody to FABP2

Research Area

Signal Transduction

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: ELISA: 1:64000, WB: 0.001 μg/ml, IHC-P: 2-3 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.IHC: In paraffin embedded Human Small Intestine shows strong staining of basement membrane and weaker staining of enterocytes. Recommended concentration, 2-3ug/ml.WB: A very strong approx 16kDa band observed in Human Duodenum lysates (calculated MW of 15.2kDa according to NP_000125.1). In transfected HEK293 transiently expressing FABP2 a band of approx. 18kDa is observed. This band is not observed in the non-transfected HEK293. Recommended concentration: 0.001 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: FABP2
• Protein Sequence: EGVEAKRIFKKD
• Molecular Weight: 15.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti FABP2 antibody, anti fatty acid binding protein 2, intestinal antibody, anti FABPI antibody, anti I-FABP antibody, anti MGC133132 antibody, anti Fatty acid-binding protein, intestinal antibody, anti intestinal fatty acid binding protein 2 antibody

HEK293 overexpressing FABP2 (RC210206) and probed with orb19465 (mock transfection in first lane), tested by Origene.

orb19465 (2.5ug/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb19465 (0.001µg/ml) staining of Human Duodenum lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

FABP2

NCBI Gene ID

2169