Gelsolin/GSN Antibody

SKU: orb19350

Description

Goat polyclonal antibody to GSN

Research Area

Musculoskeletal & Connective Tissue Research

Images & Validation

• Tested Applications: ELISA, FC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-2 μg/ml, IHC-P: 10ug/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 90kDa band observed in Human Spleen lysates (calculated MW of 85.7kDa according to NP_000168.1). Recommended concentration: 0.1-0.3 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: Gelsolin / GSN
• Protein Sequence: PRLKDKKMDAHP
• Molecular Weight: 85.7; 80.6; 81.9; 82.5; 81.5; 84.7; 83.3; 56.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti GSN antibody, anti gelsolin antibody, anti gelsolin (amyloidosis, Finnish type) antibody, anti DKFZp313L0718 antibody

Western blot analysis of Human Colorectal cancer lysate using GSN antibody

Flow cytometric analysis of HeLa cells using GSN antibody.

Quick Database Links

Gene Symbol

Gelsolin / GSN

NCBI Gene ID

2934

Kluge, Sabine et al. Monitoring changes in proteome during stepwise adaptation of a MDCK cell line from adherence to growth in suspension Vaccine, 33, 4269-4280 (2015)

PubMed: 25891398

Applications

WB

Reactivity

Canine