Goat anti-HLA-B (aa132-143) Antibody

SKU: orb318746

Description

Goat polyclonal antibody to HLA-B

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 2.5 μg/ml
• Reactivity: Human
• Application Notes:
  WB: Approx 40+45kDa bands observed in lysates of cell line Jurkat (calculated MW of 40.5kDa according to NP_005505.2). The observed 45kDa band corresponds to the glycosylated form.Recommended concentration: 0.1-0.3µg/ml. Primary incubation was 1 hour.. IHC: Paraffin embedded Human Tonsil. Recommended concentration: 2.5µg/ml.

Key Properties

• Clonality: Polyclonal
• Target: HLA-B (aa132-143)
• Protein Sequence: RLLRGHDQYAYD
• Molecular Weight: 40.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti HLA-B antibody, anti major histocompatibility complex, class I, B antibody, anti AS antibody, anti HLAB antibody, anti SPDA1 antibody, anti HLA class I histocompatibility antigen, B alpha chain antibody, anti MHC Class I HLA heavy chain antibody, anti MHC HLA-B cell surface glycoprotein antibody, anti MHC HLA-B transmembrane glycoprotein antibody, anti MHC class I antigen GN0010 antibody

Western blot analysis of Jurkat lysate using HLA-B antibody

Immunohistochemical staining of Human Tonsil using HLA-B antibody

Quick Database Links

Gene Symbol

HLA-B (aa132-143)

NCBI Gene ID

3106