Integrin beta 5 Antibody

SKU: orb19000

Description

Goat polyclonal antibody to ITGB5

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:16000, WB: 0.1-0.3 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Canine, Porcine, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 110kDa band observed in Human Ovary and Mouse Eye lysates (calculated MW of 88.1kDa according to NP_002204.2). Recommended concentration: 0.1-0.3 μg/ml. Customer feedback: observed 110kDa in Mouse Eye was confirmed and not present in knock-out mouse.

Key Properties

• Clonality: Polyclonal
• Target: Integrin beta 5
• Protein Sequence: TFNKFNKSYNGTVD
• Molecular Weight: 88.1
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti ITGB5 antibody, anti integrin, beta 5 antibody, anti FLJ26658 antibody

orb19000 (0.1ug/ml) staining of Human Ovary lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Quick Database Links

Gene Symbol

Integrin beta 5

NCBI Gene ID

3693