KLF8/BKLF3 Antibody

SKU: orb18394

Description

Goat polyclonal antibody to KLF8

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 1-3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human
• Predicted Reactivity: Porcine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 50kDa band observed in Human Kidney lysates (calculated MW of 39.3 acording to NP_009181.2). In glioblastomas this protein was reported to run at appr. 60kDa (Ding et al. J Biol Chem. 2005 Feb 25;280(8):6802-15; PMID: 15557280). Recommended for use at 1-3 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: KLF8 / BKLF3
• Protein Sequence: VDMDKLINNLEVQ
• Molecular Weight: 39.3; 27.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti KLF8 antibody, anti BKLF3 antibody, anti Kruppel-like factor 8 antibody, anti RP13-1021K9.1 antibody, anti DKFZp686O08126 antibody, anti DXS741 antibody, anti MGC138314 antibody, anti ZNF741 antibody, anti zinc finger protein 741 antibody, anti basic kruppel-like factor 3 antibody

orb18394 staining (2ug/ml) of Human Kidney lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18394 (3.75µg/ml) staining of paraffin embedded Human Small Intestine. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Quick Database Links

Gene Symbol

KLF8 / BKLF3

NCBI Gene ID

11279