Leptin Receptor Antibody

SKU: orb19243

Description

Goat polyclonal antibody to Leptin Receptor

Research Area

Metabolism Research

Images & Validation

• Tested Applications: ELISA, FC, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.1-1 μg/ml, IHC-P: 4 μg/ml
• Reactivity: Human, Mouse, Rat
• Predicted Reactivity: Canine
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:16000.WB: Approx 150kDa band observed in Human Brain lysates (calculated MW of 132kDa according to NP_002294.2). Recommended concentration: 1-3 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: Leptin Receptor
• Protein Sequence: TQDDIEKHQSDAG
• Molecular Weight: 132; 102; 109
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CD 295 antibody, anti CD295 antibody, anti LEP R antibody, anti LEP-R antibody, anti LEPR antibody, anti LEPRD antibody, anti LEPROT antibody, anti Leptin receptor antibody, anti OB R antibody, anti OB receptor antibody, anti OB-R antibody, anti obl antibody, anti Obr antibody

Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line) permeabilized with 0.5% Triton. Primary incubation 1hr (10 ug/ml) followed by Alexa Fluor 488® conjugated secondary antibody (1 ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Immunofluorescence analysis of paraformaldehyde fixed U251. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic and vesicles staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.

Immunofluorescence analysis of paraformaldehyde fixed HEK293. Primary incubation 1hr (1:50 dilution) followed by Alexa Fluor® 488 secondary antibody (1:2000 dilution), showing cytoplasmic and vesicles staining. The nuclear stain is DAPI (blue). Negative control: Anti-Goat IgG followed by Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

Leptin Receptor

NCBI Gene ID

3953