Goat anti-LTF Antibody

SKU: orb20033

Description

Goat polyclonal to LTF

Research Area

Immunology & Inflammation; Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.05-0.2 μg/ml, IHC-P: 2-4μg/ml
• Reactivity: Human
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.IHC: In paraffin embedded blood vessel shows strong staining of cytoplasm of neutrophils.. Recommended concentration, 2-4ug/ml.WB: Approx. 85kDa band observed in Human Peripheral Mononucleocyte lysates (calculated MW of 78.2kDa according to NP_002334.2). Recommended concentration: 0.05-0.2 μg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: LTF
• Protein Sequence: ENYKSQQSSDPDP
• Molecular Weight: 78.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti LTF antibody, anti lactotransferrin antibody, anti GIG12 antibody, anti HLF2 antibody, anti LF antibody, anti growth-inhibiting protein 12 antibody, anti lactoferrin antibody, anti neutrophil lactoferrin antibody, anti talalactoferrin antibody

4 µg/ml staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH6, HRP-staining.

Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

Primary incubation 1 hour at room temperature. Images A, B: Human Bone Marrow and Prostate lysate at primary Ab concentration 0.01 ug/ml. Image C: Human Spleen lysate at primary Ab concentration 0.005 ug/ml. (Loaded 35 µg protein in RIPA buffer, per lane). Detected by chemiluminescence.

Quick Database Links

Gene Symbol

LTF

NCBI Gene ID

4057