MIF Antibody

SKU: orb19028

Description

Goat polyclonal antibody to MIF

Research Area

Immunology & Inflammation

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.01-0.03 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:128000.WB: Approx 13kDa band observed in Human Lymph Node and Thymus lysates (calculated MW of 12.5kDa according to NP_002406.1). Recommended concentration: 0.01-0.03 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: MIF
• Protein Sequence: NAANVGWNNSTFA
• Molecular Weight: 12.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti acrophage migration inhibitory factor (glycosylation-inhibiting factor) antibody, anti GIF antibody, anti GLIF antibody, anti Glycosylation inhibiting factor antibody, anti Glycosylation-inhibiting factor antibody, anti L-dopachrome isomerase antibody, anti L-dopachrome tautomerase antibody, anti MIF antibody, anti MIF protein antibody, anti MIF_HUMAN antibody, anti MMIF antibody, anti Phenylpyruvate tautomerase antibody

Western blot analysis of staining of Human Thymus lysate using MIF antibody.

Western blot analysis of staining of Daudi (A) and Jurkat (B) lysate using MIF antibody.

Immunohistochemical staining of Human Thymus using MIF antibody.

Quick Database Links

Gene Symbol

MIF

NCBI Gene ID

4282