Npap60/Nup50 Antibody

SKU: orb18439

Description

Goat polyclonal antibody to NUP50

Research Area

Epigenetics

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.2-1 μg/ml, IHC-P: 2.5-3.8µg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Mouse, Rat
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 60kDa band observed in lysates of cell line Jurkat (calculated MW 50.1 kDa according to NP_009103.2). Recommended for use at 0.2-1 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: Npap60 / Nup50
• Protein Sequence: ELHKILLEKKDA
• Molecular Weight: 50.1; 46.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti NUP50 antibody, anti NPAP60 antibody, anti NPAP60L antibody, anti MGC39961 antibody, anti DKFZP564A043 antibody, anti nucleoporin 50kDa antibody, anti nuclear pore-associated protein 60L antibody, anti CTA-268H5.7 antibody, anti OTTHUMP00000199530 antibody

Western blot analysis of Jurkat lysate using NUP50 antibody

Immunohistochemical staining of Human Uterus using NUP50 antibody

Quick Database Links

Gene Symbol

Npap60 / Nup50

NCBI Gene ID

10762