NPHS2/SRN1 Antibody

SKU: orb131711

Description

Goat polyclonal to NPHS2

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:64000, WB: 0.5-2 μg/ml
• Reactivity: Human, Mouse
• Predicted Reactivity: Bovine, Porcine
• Application Notes:
  Approx 45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to NP_055440.1). Recommended concentration: 0.3-1 μg/ml.WB: Approx 45kDa band observed in Human and Mouse Kidney lysates (calculated MW of 42.2kDa according to NP_055440.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: NPHS2 / SRN1
• Protein Sequence: SPSKPVEPLNPKK
• Molecular Weight: 42.2
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

NPHS2; nephrosis 2, idiopathic, steroid-resistant (podocin); PDCN; SRN1; podocin

Western blot analysis of taining of Human (A) and Mouse (B) Kidney lysates using NPHS2 antibody.

Western blot analysis of staining of Pig Kidney lysates using NPHS2 antibody.

Western blot analysis of Human (A) and Mouse (B) Kidney lysates (35ug protein in RIPA buffer) using NPHS2 antibody

Quick Database Links

Gene Symbol

NPHS2 / SRN1

NCBI Gene ID

7827