P2rx4 (mouse) Antibody

SKU: orb12376

Description

Goat polyclonal to P2rx4.

Research Area

Cardiovascular Disease, Neuroscience

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:16000, WB: 0.3-1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human
• Predicted Reactivity: Bovine, Canine, Mouse, Porcine, Rat
• Application Notes:
  WB: Approx 48-50kDa band observed in lysates of cell line A549 (calculated MW of 43.4kDa according to Human NP_002551.2). This molecular weight is routinely observed by other sources. Recommended concentration: 0.3-1µg/ml. Primary incubation 1 hour at room te. IHC: Paraffin embedded Human Uterus. Recommended concentration: 5µg/ml.. Notes: Immunofluorescence: Strong expression of the protein seen in the cytoplasm of A431 cells. Recommended concentration: 10µg/ml.

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Key Properties

• Clonality: Polyclonal
• Target: P2rx4 (mouse)
• Protein Sequence: ETDSVVSSVTTKAK
• Molecular Weight: 43.5
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti P2rx4 antibody

Western blot analysis of human Kidney tissue using P2rx4 antibody

Immunohistochemical staining of paraffin embedded human uterus tissue using P2RX4 antibody (primary antibody at 1:200)

Quick Database Links

Gene Symbol

P2rx4 (mouse)

NCBI Gene ID

5025