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PGC1A/PPARGC1A Antibody

SKU: orb19529

Description

Goat polyclonal antibody to PPARGC1A

Research Area

Epigenetics

Images & Validation

Tested ApplicationsELISA, FC, IF, IHC
Dilution RangeELISA: 1:32000, WB: 0.5-2 μg/ml, IF/ICC: 10μg/ml, FACS: 10ug/ml
ReactivityHuman
Predicted ReactivityBovine, Canine, Mouse, Rat
Application Notes
ELISA: Peptide ELISA: antibody detection limit dilution 1:64000.WB: Approx 100kDa band observed in Rat Stomach lysates (calculated MW of 91.0kDa according to NP_037393.1. Recommended concentration: 1-3 μg/ml.

Key Properties

ClonalityPolyclonal
TargetPGC1A/PPARGC1A
Protein SequenceDGLFDDSEDESDK
Molecular Weight91.0; 91.7; 89.6; 77.1; 91.5
PurificationPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesSupplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

anti PGC1A antibody, anti PPARGC1A antibody, anti peroxisome proliferator-activated receptor gamma, coactivator 1 alpha antibody, anti LEM6 antibody, anti PGC-1(alpha) antibody, anti PGC-1v antibody, anti PGC1 antibody, anti PPARGC1 antibody, anti PPAR gamma coactivator variant form antibody, anti PPAR gamma coactivator-1 antibody, anti ligand effect modulator-6 antibody, anti peroxisome proliferative activated receptor antibody, anti gamma, coactivator 1 antibody, anti peroxisome proliferative activated receptor, gamma, coactivator 1, alpha antibody

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PGC1A/PPARGC1A Antibody

Western blot analysis of Rat Stomach lysate using PPARGC1A antibody.

PGC1A/PPARGC1A Antibody

Immunofluorescence analysis of paraformaldehyde fixed HeLa cells using PPARGC1A antibody.

PGC1A/PPARGC1A Antibody

Immunofluorescence analysis of paraformaldehyde fixed U2OS cells using PPARGC1A antibody.

PGC1A/PPARGC1A Antibody

Flow cytometric analysis of paraformaldehyde fixed HeLa cells using PPARGC1A antibody.

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

IHC
Immunohistochemistry
View Protocol
FC
Flow Cytometry
View Protocol
IF
Immunofluorescence
View Protocol
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

PGC1A/PPARGC1A Antibody (orb19529)

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Available Sizes

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100 μg
$ 560.00
Free Secondary Antibody (20 ul)0/0

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