Phenylalanine Hydroxylase Antibody

SKU: orb20657

Description

Goat polyclonal to Phenylalanine Hydroxylase

Research Area

Pharmacology & Drug Discovery

Images & Validation

• Tested Applications: ELISA, WB
• Dilution Range: ELISA: 1:32000, WB: 0.1-0.3 μg/ml, IHC-P: 3.75 μg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Bovine, Canine, Mouse
• Application Notes:
  ELISA: Peptide ELISA: antibody detection limit dilution 1:32000.WB: Approx 48kDa band observed in Human Liver lysates (calculated MW of 51.9kDa according to NP_000268.1). Recommended concentration: 0.3-1 μg/ml.

Key Properties

• Clonality: Polyclonal
• Target: Phenylalanine Hydroxylase
• Protein Sequence: ESRPSRLKKDE
• Molecular Weight: 51.9
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PAH antibody, anti phenylalanine hydroxylase antibody, anti PH antibody, anti PKU antibody, anti PKU1 antibody

orb20657 (0.3ug/ml) staining of Human Liver lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Western blot analysis of Mouse (Lane 1) and Rat (Lane 2) Liver lysate using Phenylalanine Hydroxylase antibody

Immunohistochemical staining of Human Kidney using Phenylalanine Hydroxylase antibody

Quick Database Links

Gene Symbol

Phenylalanine Hydroxylase

NCBI Gene ID

5053