PRKCB Antibody

SKU: orb107663

Description

Goat polyclonal to PRKCB

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IHC, WB
• Dilution Range: ELISA: 1:128000, WB: 0.1-0.3 μg/ml, IHC-P: 1-2µg/ml
• Reactivity: Human, Rat
• Predicted Reactivity: Canine
• Application Notes:
  WB: Approx 80kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9kDa according to NP_997700.1). Recommended concentration: 0.1-0.3µg/ml.. IHC: In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2µg/ml.. Notes: Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.

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Key Properties

• Clonality: Polyclonal
• Target: PRKCB
• Protein Sequence: DKEFTRQPVELT
• Molecular Weight: 76.9; 77.0
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti PRKCB antibody

Western blot analysis of Human Placenta lysate (35ug protein in RIPA buffer) using PRKCB antibody

Western blot analysis of Human Placenta (Lane 1) and Rat Brain (Lane 2) lysates using PRKCB antibody

Immunohistochemical staining of Human Spleen using PRKCB antibody

Quick Database Links

Gene Symbol

PRKCB

NCBI Gene ID

5579