Goat anti-S100A8 Antibody

SKU: orb12338

Description

Goat polyclonal antibody to S100A8

Research Area

Cell Biology

Images & Validation

• Tested Applications: ELISA, IF, IHC, WB
• Dilution Range: ELISA: 1:32000, WB: 0.03-0.1 μg/ml, IHC-P: 5 μg/ml
• Reactivity: Human

Key Properties

• Clonality: Polyclonal
• Target: S100A8
• Protein Sequence: KGADVWFKELDINT
• Molecular Weight: 10.8
• Purification: Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
• Conjugation: Unconjugated

Storage & Handling

• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Expiration Date: 12 months from date of receipt.
• Disclaimer: For research use only

Alternative Names

anti CAGA antibody, anti Calgranulin-A antibody, anti CFAG antibody, anti CGLA antibody, anti Chemotactic cytokine CP-10 antibody, anti CP-10 antibody, anti Cystic fibrosis antigen antibody, anti L1Ag antibody, anti Leukocyte L1 complex light chain antibody, anti MA387 antibody, anti Migration inhibitory factor-related protein 8 antibody, anti Myeloid-related protein 8 antibody, anti Neutrophil cytosolic 7 kDa protein antibody, anti NIF antibody, anti Pro-inflammatory S100 cytokine antibody, anti Protein S100-A8 antibody, anti S100 calcium binding protein A8 (calgranulin A) antibody, anti S100 calcium binding protein A8 antibody, anti S100A8 antibody, anti S100A8/S100A9 complex, included antibody, anti Urinary stone protein band A antibody, anti MRP8 antibody, anti MRP-8 antibody, anti 60B8Ag antibody

5 µg/mL staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic/extracellular staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

Quick Database Links

Gene Symbol

S100A8

NCBI Gene ID

6279